Evaluation of the toxicity of Araribá (Centrolobium tomentosum) using brine Shrimp test

  • Roberto Carlos de Sá Silva Universidade de Taubaté
  • Julio Cesar Raposo de Almeida Programa de Pós-graduação em Ciências Ambientais, UNITAU
  • Ana Aparecida da Silva Almeida Universidade de Taubaté (UNITAU)
  • Gokithi Akisue Faculdade de Pindamonhangaba
  • Matheus Diniz Gonçalves Coelho Faculdade de Pindamonhangaba
  • José Roberto Pereira APTA - Pindamonhangaba
Keywords: phytotherapeutic, drugs, tannins, brine shrimp

Abstract

Since the dawn of medicine, man has used natural products for the treatment of disease. There has been a recent increase in studies of the therapeutic characteristics plants in popular use in many places of the world. In this context, the species Centrolobium tomentosum, Fabaceae, known popularly as Araribá or Araruva, is used in Brazilian folk medicine as an astringent for wound treatment and bruises due to the large amount of tannins contained in the bark. This work used toxicity tests to assess the biological activity of ethanolic extracts from C. tomentosum with the specific objectives of obtaining concentrated extracts from the bark and wood of this species and determining the total phenols and tannins present in these extracts. We collected araribá samples in order to obtain ethanolic extracts through the percolation process. We then made a qualitative chemical identification of hydrolysable tannins and condensed tannins. We used the Folin-Ciocalteu method for the phenols quantification and the casein precipitation method for the tannins determination. The toxicity of extracts was evaluated using the brine shrimp bioassay (Artemia salina), in which the C. tomentosum bark extract showed moderate toxicity, with estimated LC50 = 416 μg.ml-1, whereas the leaves and wood extracts of this species showed low toxicity with LC50 = 537 μg.ml-1 and 826 μg.ml-1, respectively.

Author Biography

Roberto Carlos de Sá Silva, Universidade de Taubaté
graduação em Ciências Biológicas pela Universidade Estadual Paulista Júlio de Mesquita Filho (1986), mestrado em Ciências Biológicas (Botânica) pela Universidade Estadual Paulista Júlio de Mesquita Filho (1991) e doutorado em Agronomia (Solos e Nutrição de Plantas) pela Universidade de São Paulo (1997). Atualmente é professor assistente doutor da Universidade de Taubaté, exercendo cargo de Pró-reitora de Extensão e Relações Comunitárias. Tem experiência Nutrição e Crescimento Vegetal, atuando principalmente nos seguintes temas: nutrição mineral, análise de crescimento vegetal, crescimento vegetal, estresse mineral e germinação. (Texto informado pelo autor)
Published
19/08/2014
Section
Special